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tmb chromogen solution for blotting  (Beyotime)


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    Structured Review

    Beyotime tmb chromogen solution for blotting
    Tmb Chromogen Solution For Blotting, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tmb+chromogen+solution/tmb+chromogen+solution/pmc12205366-177-4-8
    Average 90 stars, based on 1 article reviews
    tmb chromogen solution for blotting - by Bioz Stars, 2026-10
    90/100 stars

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    Incubation:

    Article Title: Establishment of a Direct Competitive ELISA for Camel FGF21 Detection
    Article Snippet: .. We added 50 μL standard protein diluents, with gradient concentrations of 10000, 2000, 400, 80, 16, 3.2, 0.64, and 0.128 ng/mL, or sample to be tested into each well of coating plate together with 50 μL Biotin-camel FGF21 for incubation at 37 °C for 1 h. After washing 3 times with PBST, HRP-conjugated avidin antibody was added into each well and incubated at 37 °C for 30 min. After washing with PBST 5 times, TMB chromogen solution (Beyotime, Beijing, China) was added into each well and maintained for 15 min. After adding stop solution for TMB substrate (Beyotime, Beijing, China) in each well, an OD value of 450 nm wavelength was recorded under the microplate reader. .. Fitting curves to establish equations of the relationship between the negative logarithm of camel FGF21 antigen content and OD450 value were made using GraphPad Prism 9 software.

    Article Title: Single-dose intranasal AdC68-vectored vaccines rapidly protect Syrian hamsters against lethal Nipah virus infection.
    Article Snippet: The plates were incubated for 1 hour at 37°C, washed four times 555 with PBST (0.1% Tween 20 in 1 PBS), and then incubated with horseradish peroxidase 556 (HRP)-conjugated goat anti-mouse IgG (Abcam, ab6789; diluted 1:20,000) or IgA (Abcam, 557 ab97235, diluted 1:10,000) or HRP-conjugated goat anti-hamster IgG (Abcam, ab6892, 558 diluted 1:15,000) in PBS with 5% skim milk for 1.5 hours at 37°C. .. Following incubation, 559 the plates were washed 4 times with PBST and developed with TMB chromogen solution 560 (Beyotime, P0209) for 15 minutes. .. The reactions were stopped by adding stop solution for 561 TMB (Beyotime, P0215).

    Article Title: Mesomycoplasma hyopneumoniae lipoprotein Mhp390 serves as a plasminogen receptor mediating extracellular matrix degradation and respiratory epithelial cells injury.
    Article Snippet: .. The plates were washed with PBST to eliminate non-adherent proteins, followed by the addition of a His-tag monoclonal antibody (Proteintech, USA, Cat No. 66005) into each well and incubation at 37 °C for 60 min. After further washing with PBST, horseradish peroxidase (HRP)-conjugated goat anti-mouse antibody (Proteintech, USA, Cat No. SA00001) was added to the wells and incubated at 37 °C for 60 min. After washing with PBST, the wells were subsequently exposed to TMB chromogen solution (Beyotime, China, Cat No. P0209) and stopped with stop solution (Beyotime, China, Cat No. P0215). ..

    Article Title: Establishment of a Direct Competitive ELISA for Camel FGF21 Detection.
    Article Snippet: .. We added 50 μL standard protein diluents, with gradient concentrations of 10000, 2000, 400, 80, 16, 3.2, 0.64, and 0.128 ng/mL, or sample to be tested into each well of coating plate together with 50 μL Biotin-camel FGF21 for incubation at 37 ◦C for 1 h. After washing 3 times with PBST, HRP-conjugated avidin antibody was added into each well and incubated at 37 ◦C for 30 min. After washing with PBST 5 times, TMB chromogen solution (Beyotime, Beijing, China) was added into each well and maintained for 15 min. After adding stop solution for TMB substrate (Beyotime, Beijing, China) in each well, an OD value of 450 nm wavelength was recorded under the microplate reader. .. Fitting curves to establish equations of the relationship between the negative logarithm of camel FGF21 antigen content and OD450 value were made using GraphPad Prism 9 software.

    Avidin-Biotin Assay:

    Article Title: Establishment of a Direct Competitive ELISA for Camel FGF21 Detection
    Article Snippet: .. We added 50 μL standard protein diluents, with gradient concentrations of 10000, 2000, 400, 80, 16, 3.2, 0.64, and 0.128 ng/mL, or sample to be tested into each well of coating plate together with 50 μL Biotin-camel FGF21 for incubation at 37 °C for 1 h. After washing 3 times with PBST, HRP-conjugated avidin antibody was added into each well and incubated at 37 °C for 30 min. After washing with PBST 5 times, TMB chromogen solution (Beyotime, Beijing, China) was added into each well and maintained for 15 min. After adding stop solution for TMB substrate (Beyotime, Beijing, China) in each well, an OD value of 450 nm wavelength was recorded under the microplate reader. .. Fitting curves to establish equations of the relationship between the negative logarithm of camel FGF21 antigen content and OD450 value were made using GraphPad Prism 9 software.

    Article Title: Establishment of a Direct Competitive ELISA for Camel FGF21 Detection.
    Article Snippet: .. We added 50 μL standard protein diluents, with gradient concentrations of 10000, 2000, 400, 80, 16, 3.2, 0.64, and 0.128 ng/mL, or sample to be tested into each well of coating plate together with 50 μL Biotin-camel FGF21 for incubation at 37 ◦C for 1 h. After washing 3 times with PBST, HRP-conjugated avidin antibody was added into each well and incubated at 37 ◦C for 30 min. After washing with PBST 5 times, TMB chromogen solution (Beyotime, Beijing, China) was added into each well and maintained for 15 min. After adding stop solution for TMB substrate (Beyotime, Beijing, China) in each well, an OD value of 450 nm wavelength was recorded under the microplate reader. .. Fitting curves to establish equations of the relationship between the negative logarithm of camel FGF21 antigen content and OD450 value were made using GraphPad Prism 9 software.

    Purification:

    Article Title: Construction of a Plasmid-Free Escherichia coli Strain with Enhanced Heme Supply to Produce Active Hemoglobins
    Article Snippet: .. The peroxidase activities of purified proteins were detected using TMB Chromogen Solution (Beyotime Biotech, Shanghai, China). ..

    Article Title: Construction of a Plasmid-Free Escherichia coli Strain with Enhanced Heme Supply to Produce Active Hemoglobins.
    Article Snippet: .. The peroxidase activities of purified proteins were detected using TMB Chromogen Solution (Beyotime Biotech, Shanghai, Metabolites 2025, 15, 151 9 of 23 China). ..

    other:

    Article Title: Toll Like Receptors Promote High Glucose-Induced Vascular Endothelial Cell Dysfunction by Regulating Neutrophil Extracellular Traps Formation.
    Article Snippet: TMB Chromogen Solution, Stop Solution for TMB Substrate,CCK-8 assay were purchased from Beyotime (Shanghai, China).

    Article Title: Toll like receptors promote high glucose-induced vascular endothelial cell dysfunction by regulating neutrophil extracellular traps formation
    Article Snippet: TMB Chromogen Solution, Stop Solution for TMB Substrate were purchased from Beyotime (Shanghai, China).



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